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jts / nanopolish

Signal-level algorithms for MinION data
MIT License
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The output file of eventaligh is so large and no new information is written when it is 4TB #1103

Open Dongxu-Zheng opened 1 year ago

Dongxu-Zheng commented 1 year ago

Hi there, thanks for developing nanopolish for ONT data analysis! I am working on the direct RNA sequencing data and would like to use nanopolish to align the events. I noticed some things are strange:

  1. when I use multiple cores to run nanopolish event alignment, I always get the segmentation fault and my task is killed. Now I can only use one thread to run it.
  2. the output file of the event alignment is so large. Each of them is more than 4TB. I have no idea if this is normal or not.
  3. When the size of the output files reaches 4398046511104, no new information is written into the output file. But my task is still running. All of my samples have the same issue. I have no clues how to figure it out.

Many thanks for any suggestions and advice!

I installed nanopolish via Git Hub. The version number is 0.14.0

Best, Dongxu