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jts / nanopolish

Signal-level algorithms for MinION data
MIT License
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Is it feasible to combine runs basecalled with different models? #1107

Closed weishwu closed 1 year ago

weishwu commented 1 year ago

I have the following runs that were basecalled using different guppy models. Could I simply concatenate their fastqs and fast5s for methylation calling using Nanopolish?

run sample base_call_model
4211P A-1 dna_r10.4.1_e8.2_400bps_hac@v4.2.0
4092P A-1 dna_r10.4.1_e8.2_400bps_hac@v3.5.2
4031P A-1 2021-05-05_dna_r9.4.1_promethion_384_dd219f32
4212P B-2 dna_r10.4.1_e8.2_400bps_hac@v4.2.0
4093P B-2 dna_r10.4.1_e8.2_400bps_hac@v3.5.2
jts commented 1 year ago

Hi @weishwu,

Nanopolish does not support R10.4 data. I suggest you use the dorado or guppy methylation calls.

Jared

weishwu commented 1 year ago

OK. Thanks!