Open timtreis opened 5 months ago
Also from the paper:
So to be very explicit-- are the no-guide and non-target (EXCLUDING DMSO) both used as the negative controls for the CRISPR plates?
Hi all, I apologize for the delayed response. The paragraph shared by @yugeji is correct. I should update the text shared by @timtreis (thank you for catching that!).
We use both no-guide
and non-targeting guide
wells as negative control in our analyses (we don't use DMSO
).
Please let me know if you have other questions.
Okay, so to summarize, each CRISPR plate should contain:
Given this, would it be possible to share an updated layout @niranjchandrasekaran ? The reason we brought this up is because we absolutely could not find anything labeled "DMSO" on the CRISPR plates.
Thanks very much in advance!
Hi @yugeji, if you use both compounds.csv.gz
and crispr.csv.gz
, along with well.csv.gz
(all in the metadata
folder), you will find the poscon compounds and DMSO wells. For example, when the run the following
well_df = pd.read_csv('metadata/well.csv.gz')
compound_df = pd.read_csv('metadata/compound.csv.gz')
crispr_df = pd.read_csv('metadata/crispr.csv.gz')
merged_df = (
well_df.query("Metadata_Plate == 'CP-CC9-R1-03'")
.merge(crispr_df, on="Metadata_JCP2022", how="left")
.query(
"Metadata_Symbol=='PLK1' or Metadata_NCBI_Gene_ID.isna()"
)
.merge(compound_df, on="Metadata_JCP2022", how="left")
.assign(
perturbation_id=lambda x: np.where(
x.Metadata_Symbol.notna(), x.Metadata_Symbol, x.Metadata_InChIKey
)
)
)
merged_df.perturbation_id.value_counts()
I get
no-guide 11
non-targeting 10
IAZDPXIOMUYVGZ-UHFFFAOYSA-N 8
SRVFFFJZQVENJC-UHFFFAOYSA-N 4
PLK1 4
IHLVSLOZUHKNMQ-UHFFFAOYSA-N 4
IVUGFMLRJOCGAS-UHFFFAOYSA-N 4
OINGHOPGNMYCAB-UHFFFAOYSA-N 4
GJFCONYVAUNLKB-UHFFFAOYSA-N 4
LOUPRKONTZGTKE-UHFFFAOYSA-N 4
KPBNHDGDUADAGP-UHFFFAOYSA-N 4
CQKBSRPVZZLCJE-UHFFFAOYSA-N 4
Name: perturbation_id, dtype: int64
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
is DMSO while the other InChIKey
correspond to the 8 compounds poscons
.
I should note that the number of wells in your comment is for the “median” plate. Some plates have more number of wells.
Hey everyone, we're trying to compare data across "perturbation-universes". For this, we're having a deeper look into the controls in the CRISPR plates.
Their layout is, for example, like this:![image](https://github.com/jump-cellpainting/datasets/assets/12818242/1f915337-f847-410c-8eda-7c7b096c0a62)
Chandrasekaran et al. states that:
By deduction, that'd mean that
no-guide
is DMSO. However, DMSO would be a bad control for CRISPR, since the guide RNAs, unlike drugs, are not dissolved in it but rather some transfection agent. Were these acquired to stay comparable to the other drug plates?@yugeji