Closed dktanwar closed 3 years ago
Hi Deepak,
No, a G nucleotide was added to all the 5′-end of histidine tRNAs. Please read the references: Nat Methods. 2015 Sep;12(9):879-84. doi: 10.1038/nmeth.3508; Proc Natl Acad Sci U S A. 1982 Nov;79(21):6475-9. doi: 10.1073/pnas.79.21.6475.
Hi,
I got another question about the tRNA Histidine. https://github.com/junchaoshi/sports1.1#trna_db_processingpl
You mentioned that a G is added to the 5' of the sequence.
If I check the sequences, a G is already present at the start of the sequence: http://gtrnadb.ucsc.edu/genomes/eukaryota/Mmusc10/mm10-tRNAs.fa
So, I guess, you are adding a G only if the sequence does not start with G. Is this correct?
Also, is there any reference for this?
Thank you in advance for clarifying.