Adding G to tRNA Histidine

junchaoshi / sports1.1

Small non-coding RNA annotation Pipeline Optimized for rRNA- and tRNA-Derived Small RNAs

GNU General Public License v3.0

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Adding G to tRNA Histidine #20

Closed dktanwar closed 3 years ago

dktanwar commented 3 years ago

Hi,

I got another question about the tRNA Histidine. https://github.com/junchaoshi/sports1.1#trna_db_processingpl

You mentioned that a G is added to the 5' of the sequence.

If I check the sequences, a G is already present at the start of the sequence: http://gtrnadb.ucsc.edu/genomes/eukaryota/Mmusc10/mm10-tRNAs.fa

So, I guess, you are adding a G only if the sequence does not start with G. Is this correct?

Also, is there any reference for this?

Thank you in advance for clarifying.

junchaoshi commented 3 years ago

Hi Deepak,

No, a G nucleotide was added to all the 5′-end of histidine tRNAs. Please read the references: Nat Methods. 2015 Sep;12(9):879-84. doi: 10.1038/nmeth.3508; Proc Natl Acad Sci U S A. 1982 Nov;79(21):6475-9. doi: 10.1073/pnas.79.21.6475.