Closed ceidashi8 closed 9 months ago
Hi, Could you please post the entire command you used when running the SPORTS? It appears that the correct absolute or relative address for the miRBase reference was not entered. I always recommend using the absolute path of the file or folder to avoid any confusion. For instance, the absolute path could be: /home/Genome/Arabidopsis_thaliana/miRBase_21/miRBase_21-ath.
I hope this information is helpful.
Best regards, Junchao
Thank you,I took your advice to run the Sports successfully!
---Original--- From: @.> Date: Tue, Nov 14, 2023 11:09 AM To: @.>; Cc: @.**@.>; Subject: Re: [junchaoshi/sports1.1] Bowtie problem (Issue #37)
Hi, Could you please post the entire command you used when running the SPORTS? It appears that the correct absolute or relative address for the miRBase reference was not entered. I always recommend using the absolute path of the file or folder to avoid any confusion. For instance, the absolute path could be: /home/Genome/Arabidopsis_thaliana/miRBase_21/miRBase_21-ath.
I hope this information is helpful.
Best regards, Junchao
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Dear shi,when i can not match to index of bowtie-build,although I already have these index file.The error is that:
match to miRNA-match_genome Could not locate a Bowtie index corresponding to basename "Arabidopsis_thaliana/miRBase_21/miRBase_21-ath" Command: bowtie --wrapper basic-0 -f -v 0 -a -p 1 --fullref --norc --al /home/cuimingyang/sports-sRNA/text/1_S1_1_trimmed/S1_1_trimmed_match_miRNA_match_genome.fa --un /home/cuimingyang/sports-sRNA/text/1_S1_1_trimmed/S1_1_trimmed_unmatch_miRNA_match_genome.fa Arabidopsis_thaliana/miRBase_21/miRBase_21-ath /home/cuimingyang/sports-sRNA/text/1_S1_1_trimmed/S1_1_trimmed_match_genome.fa