Thank you for developing the tool. I used the provided Mus musculus pre-compiled databases files and I realized they are not the latest release. I want to use the updated files and for that I manually downloaded the mm39-tRNA fasta file. I then saw that you provide a script tRNA_db_processing.pl that adds CCA to 3'end and G to 5'end of Histidine. However, I saw that in the recommended command line you don't indicate the name of the tRNA_CCA index files, even though the bowtie index files are included in the mm10 pre-compiled database:
Hello,
Thank you for developing the tool. I used the provided Mus musculus pre-compiled databases files and I realized they are not the latest release. I want to use the updated files and for that I manually downloaded the mm39-tRNA fasta file. I then saw that you provide a script
tRNA_db_processing.pl
that adds CCA to 3'end and G to 5'end of Histidine. However, I saw that in the recommended command line you don't indicate the name of the tRNA_CCA index files, even though the bowtie index files are included in the mm10 pre-compiled database:sports.pl [...] -t /foo/bar/Mus_musculus/GtRNAdb/mm10/mm10-tRNAs
Instead of:sports.pl [...] -t /foo/bar/Mus_musculus/GtRNAdb/mm10/mm10-tRNAs_CCA
I wonder what to use with the updated files that I downloaded. Should I pass the *_CCA indexes to
sports.pl
?Thanks,
Sergio