localize via command-line + mle fitting: sx == sy

jungmannlab / picasso

A collection of tools for painting super-resolution images

https://picassosr.readthedocs.io/en/latest/?badge=latest

MIT License

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localize via command-line + mle fitting: sx == sy #482

Closed MariusGlg closed 2 months ago

MariusGlg commented 3 months ago

Picasso version: 0.6.11
Python version: 3.10.13
Operating System: Windows

Description

The Point Spread Function width in camera pixels is equal in x & y (sx == sy) when using the command line for localization + mle as the fitting option. This is not the case when using mle fitting in the picasso render module (see file attached) cmd localize

What I Did

picasso localize "filepath" -b 7 -a mle -g 150000 -r 165 122 267 227 -bl 200

rafalkowalewski1 commented 3 months ago

Hello Marius,

Thank you for pointing this out. I will fix this at the new update.

Bests, Rafal

rafalkowalewski1 commented 2 months ago

Hello Marius,

I decided that the CMD version should output different sigmas for x and y (this can be useful for filtering spots that have too different values later). The update will be available next week.

Bests, Rafal