Investigate Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4 gene

[SULTAN-ALHARBI]

• the sequence of Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4 is located here: oct4 sequence
• please use the sequence (Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4) to identify the following:
  • multiple sequence alignment and analysis of phylogenetic relationships
  • prediction of the secondary and 3D structure of Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4
  • physical and chemical properties of Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4
• you can edit the draft paper of Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4 : link to oct4 draft
• your job include in silico analysis only as follows:
  • [X] generating results
  • [ ] writing results
  • [ ] writing methods
  • [X] writing introduction
  • Deadline: 1 November 2017
• we will help you writing the manuscript

[SULTAN-ALHARBI]

Oct4_mRNA_ORF.pdf

[MManee]

• sorry mariam about this switch to oct4 gene
• as sultan mentioned in his email, we already have its primers and anti-body which will save us a lot of time and give us a better chance to complete the whole work in a short period of time

[malsaeed]

No problem at all dr.manee it's just I was hope to finish some work before I leave

No problem and we will see both genes after the break

Thank so much

[MManee]

Thank you for your understanding, you have to focus on oct4 gene only from now on.

[malsaeed]

Hello

this is the result I got

multiple sequence alignment and analysis of phylogenetic relationships

prediction of the secondary and 3D structure of Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4

8330c07e-9ea3-11e7-9685-00163e110593.POU5F1.psipred.pdf

physical and chemical properties of Camelus_dromedarius_POU_class_5_homeobox_1_POU5F1_Oct4

I will focus now on the domains

Thank you

[malsaeed]

@SULTAN-ALHARBI

Good evening

First draft trees

I like the first tree more WAITING for your response to make more changes in the tree display and format thanks you

[SULTAN-ALHARBI]

Dear @malsaeed I completely agree with you the first tree has more chance to interpretation. So we will depend on the first tree for oct4 paper the next step is to explain this tree in the result section Good luck

[malsaeed]

Dear @MManee @SULTAN-ALHARBI

hope you all doing will

I need approval to add the figures to sharelatex

Thank you

Mariam

[SULTAN-ALHARBI]

Hi @malsaeed After you uploading your figure, use this \begin{figure}[H] \centering \includegraphics[width=100mm, height=80mm]{figures/Oct4figures/Oct4_PhyloTree.png} \caption{Example of figure} \label{fig:Oct4PhyloTree_REsults} \end{figure}

[atalgarni]

Dear @malsaeed hope you are doing well..... I edited the amino acid homology table as you have notice early this morning.... we do't have a Geneious license at the moment so we gonna have to ask you to do multiple alignment using the sequences listed in the edited table..... please refer to Table 1. on the latex version.

@amer1404

[malsaeed]

Dear @atalgarni

I’m fine hope you doing fine too

I will do it tomorrow inshallah

Thanks so much

[malsaeed]

@atalgarni

This is the new multiple alignment but Two hump camel protein length is 340 and in the table is 309 ,Do you think there is something wrong or not

thank you