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stepwise_mol_bio
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Add a plate-pouring protocol
#19
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kalekundert
opened
2 years ago
kalekundert
commented
2 years ago
This would include:
How to prepare the LB-agar, e.g. mix all ingredients manually, use premixes, which recipe to use, etc.
Autoclave parameters, e.g. increase autoclave time based on volume
Here's a table:
https://lab.vanderbilt.edu/wp-content/uploads/sites/21/public_files/101813_How%20long%20do%20I%20autoclave.pdf
This page (which links to the above table) also mentions that adding water to the bottom of the autoclave container can help with even heating:
https://lab.vanderbilt.edu/cdb-core-lab/2018/05/24/how-long-do-i-autoclave-liquids/
When to add antibiotics, how much antibiotic to add, etc.
How much to pour for each plate, e.g. 25 mL for 100 mm plate, but maybe more for longer-term storage or less for shorter.
Options to skip the antibiotic/plate-pouring steps, e.g. to make pre-sterilized LB-agar that can be melted and poured later.
This would include: