Add "unbiased" PCR protocol

kalekundert / stepwise_mol_bio

MIT License

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Open kalekundert opened 2 years ago

kalekundert commented 2 years ago

The gist of the protocol is:

Run a small-scale qPCR reaction to see how many cycles can be done before amplification in each cycle is no longer saturated
Do a large-scale PCR for that many cycles.