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kalekundert / stepwise_mol_bio

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digest: add phosphatase option #39

Open kalekundert opened 2 years ago

kalekundert commented 2 years ago

NEB Quick CIP can be added directly to restriction digestion reactions: https://www.neb.com/protocols/2019/06/04/protocol-for-dephosphorylation-of-5-ends-of-dna-using-quick-cip-neb-m0525

kalekundert commented 2 years ago

This could also be it's own protocol; I'll have to think about which would be best.

kalekundert commented 1 year ago

Maybe it would be best just to treat QuickCIP as another enzyme that you could add to the reaction. That would be nice an unobtrusive.

I'd need a way to specify custom enzymes, but I should have that anyways. It should be a section in the stepwise config, e.g. [molbio.digest.enzyme.<name>]. The global config could contain an entry for QuickCIP. The enzymes loaded from NEB would be a fallback after the stepwise enzymes are searched.