Closed kapsakcj closed 4 years ago
Currently the gpu-basecalling script only checks for native barcodes 1-12 upon using ligation and yes barcoding options.
ligation
yes
In 01_basecall-w-gpu.sh:
01_basecall-w-gpu.sh
guppy_basecaller [more options] \ --barcode_kits "EXP-NBD104"
This should make guppy look for all native barcodes 1-24:
guppy_basecaller [more options] \ --barcode_kits "EXP-NBD104 EXP-NBD114"
according to these directions: https://community.nanoporetech.com/protocols/Guppy-protocol/v/gpb_2003_v1_revq_14dec2018/barcoding-demultiplexing
For future reference:
Should have checked before I published the v0.4.2 release 🤦
Currently the gpu-basecalling script only checks for native barcodes 1-12 upon using
ligation
andyes
barcoding options.In
01_basecall-w-gpu.sh
:This should make guppy look for all native barcodes 1-24:
according to these directions: https://community.nanoporetech.com/protocols/Guppy-protocol/v/gpb_2003_v1_revq_14dec2018/barcoding-demultiplexing
For future reference:
Should have checked before I published the v0.4.2 release 🤦