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kdkorthauer / dmrseq

R package for Inference of differentially methylated regions (DMRs) from bisulfite sequencing
MIT License
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How to combinde several samples with different dimensions ? #60

Closed DengEr-1993 closed 7 months ago

DengEr-1993 commented 7 months ago

Hello there,

I have several RRBS CpG results samples (CpG.txt format)which have different dimensions.

In order to do some analysis based on dmrseq, I have to convert them into Bsseq format files and then combine them together.

There are many methods to combine them together, for example:

  1. combine() from bsseq
  2. read.bismark() from dmrseq
  3. makeBSseqDat() from DSS

But all of them need methylation matrix with the same dimensions.

Actually, samples from the same or different groups have their own numbers of CpGs.

So I believe there must be better method to combine sample together.

Could you give me some advice on this step ? Or what I thought is wrong ?

Thank you very much.

kdkorthauer commented 7 months ago

Hi @DengEr-1993,

As none of these functions are from dmrseq (read.bismark() is actually a bsseq function), I'd suggest you refer to the documentation from those packages, and reach out to the respective maintainers if you're still stuck. Thanks!

DengEr-1993 commented 6 months ago

@kdkorthauer Sorry for that I made mistakes. Thanks anyway.