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kevinblighe / EnhancedVolcano

Publication-ready volcano plots with enhanced colouring and labeling
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Can natural log (not Log2FC) also be used equally well? #49

Closed CodeInTheSkies closed 4 years ago

CodeInTheSkies commented 4 years ago

Hi Kevin,

I just came across your package, and am about to try it. Looks very exciting! Before that, I just wanted to double check the following. My DE list is actually from single-cell data, specifically from the Seurat package. Therefore, they are not log2-based FCs, but rather based on the natural log. Does this matter in any way? As far as I can see, it shouldn't, but I wanted to ask you, is the package more optimal for Log2FCs in any way? Hope I can still benefit from the package!

Also, are there any points/tips to keep in mind when using the package for single-cell data?

Many thanks!

kevinblighe commented 4 years ago

Hey, yes, you can technically use any type of fold change, even linear (unlogged) fold changes. You would obviously have to change the x-axis legend, though!

Kevin