search

kevinkovalchik / RawTools

RawTools is an open-source and freely available package designed to perform scan data parsing and quantification, and quality control analysis of Thermo Orbitrap raw mass spectrometer files from data-dependent acquisition experiments.
Apache License 2.0
64 stars 19 forks source link

未经处理的异常: System.ComponentModel.Win32Exception: 拒绝访问 #27

Closed marcos914 closed 2 years ago

marcos914 commented 5 years ago

Describe the bug When I run the last command with identipy， RawTools qc -d G:\RENZHE\rawtools -q G:\RENZHE\rawtools\qc_output_wSearch\ -i --db G:\RENZHE\rawtools\uniprot_human.fasta --fmods 57.0214@C --xmod 15.9949@M -N 1000 Checking Identipy dependencies The following error occurred：System.ComponentModel.Win32Exception: 拒绝访问， Although I can use xtandem for quality control

kevinkovalchik commented 5 years ago

Hello,

Have you tried using -P and -I to specify the locations of the Python executable and the IdentiPy script? For example:

RawTools -qc -d C:\MyRawFiles -q C:\MyRawFiles\QcOutput
-s identipy -P C:\Python27\python.exe -I C:\Python27\Scripts\identipy-script.py --db C:\MyRawFiles\uniprot_human.fasta

Kevin