khanlab / SPIMprep

A Snakemake workflow for pre-processing single plane illumination microscopy (SPIM, aka lightsheet microscopy)
MIT License
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Alternate workflow for data that is already stitched (prestitched) #33

Closed akhanf closed 4 months ago

akhanf commented 5 months ago

This sets up an alternate prestitched workflow for data that are already stitched, that only performs the conversion to OME-Zarr. The main workflow is now denoted as the blaze workflow.

The type of workflow to run is indicated by the acquisition flag in the datasets.tsv file, if it includes the keyword somewhere. Note: this also sets us up to customize for any other acquisitions (since the metadata grabbing was very specific to the blaze microscope)..

This PR also includes a change to 1x4096x4096 chunk sizes (1x1024x1024 resulted in ~1.5MB chunks, too small)