I am getting the following error when running the paired-end alignment command provided in your markup file. It's c;learly a SNAP error but I want to know if there's a way to bypass or increase memory allocation.
Thank you
Loading index from directory... 0s. 60856842 bases, seed size 20
Aligning.
BigAllocator: allocating too much memory, 6329296 > 6329252
SNAP exited with exit code 1 from line 489 of file SNAPLib/BigAlloc.cpp
(None, None)
BS-seeker3 Result
Final Alignment Report
================================================
Number of reads in total: 20000
Number of unique-hits reads (before post-filtering): 0.0
Number of reads mapped after post-filtering 0.0
Alignment Time: 0.88127207756secs
Final Cytosine Report
================================================
Total Number of Cytosines: 0.0
Total Number of Cs in CpG context: 0.0
Total Number of Cs in CHG context: 0.0
Total Number of Cs in CHH context: 0.0
Rate of Methylation
mCG 0.000%
mCHG 0.000%
mCHH 0.000%
I am getting the following error when running the paired-end alignment command provided in your markup file. It's c;learly a SNAP error but I want to know if there's a way to bypass or increase memory allocation. Thank you
command Used: ./bs3-align -1 test_data/pair1.fq -2 test_data/pair2.fq -o WGBC -f sam -g reference_genome/genome.fa -d reference_genome/
Error:
BS-Seeker3 beta
Welcome to SNAP version 1.0beta.18.
Loading index from directory... 0s. 60856842 bases, seed size 20 Aligning. BigAllocator: allocating too much memory, 6329296 > 6329252 SNAP exited with exit code 1 from line 489 of file SNAPLib/BigAlloc.cpp (None, None)
================================================
Number of reads in total: 20000 Number of unique-hits reads (before post-filtering): 0.0 Number of reads mapped after post-filtering 0.0
Alignment Time: 0.88127207756secs
================================================
Total Number of Cytosines: 0.0 Total Number of Cs in CpG context: 0.0 Total Number of Cs in CHG context: 0.0 Total Number of Cs in CHH context: 0.0
Rate of Methylation mCG 0.000% mCHG 0.000% mCHH 0.000%