khuang28jhu / bs3

BS-Seeker3: An Ultra-fast, Versatile Pipeline for Mapping Bisulfite-treated Reads.
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BigAllocator: allocating too much memory #32

Open Ckhyriem opened 4 years ago

Ckhyriem commented 4 years ago

I am getting the following error when running the paired-end alignment command provided in your markup file. It's c;learly a SNAP error but I want to know if there's a way to bypass or increase memory allocation. Thank you

command Used: ./bs3-align -1 test_data/pair1.fq -2 test_data/pair2.fq -o WGBC -f sam -g reference_genome/genome.fa -d reference_genome/

Error:

BS-Seeker3 beta

Welcome to SNAP version 1.0beta.18.

Loading index from directory... 0s. 60856842 bases, seed size 20 Aligning. BigAllocator: allocating too much memory, 6329296 > 6329252 SNAP exited with exit code 1 from line 489 of file SNAPLib/BigAlloc.cpp (None, None)

    BS-seeker3 Result

    Final Alignment Report

================================================

Number of reads in total: 20000 Number of unique-hits reads (before post-filtering): 0.0 Number of reads mapped after post-filtering 0.0

Alignment Time: 0.88127207756secs

    Final Cytosine Report

================================================

Total Number of Cytosines: 0.0 Total Number of Cs in CpG context: 0.0 Total Number of Cs in CHG context: 0.0 Total Number of Cs in CHH context: 0.0

Rate of Methylation mCG 0.000% mCHG 0.000% mCHH 0.000%

twelvesummer commented 4 years ago

+1

kachelo commented 3 years ago

Upgrade SNAP to version 1.0.4

Enjoy