kids-first / kf-alignment-workflow

:microscope: Alignment workflow for Kids-First DRC
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As qc wf #115

Closed sickler-alex closed 3 years ago

sickler-alex commented 3 years ago

Description

Please include a summary of the change and which issue is fixed. Please also include relevant motivation and context. List any dependencies that are required for this change.

Changes to the FastQC workflow to fail the workflow if an input file fails in FastQC and calculate Q20 and Q30 on multiple input files.

Fixes # https://app.zenhub.com/workspaces/d3b-bfx-5cdd720eed0dce4209e0b8a5/issues/d3b-center/bixu-tracker/931

Type of change

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How Has This Been Tested?

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Test Configuration:

Checklist:

sickler-alex commented 3 years ago

The first test link failed because the one input file failed in FastQC. The job.err log file shows two errors while running FastQC and another one at the end saying that there was a problem when running FastQC which is expected. The first error is an error with the FastQC parameter file when reading the duplication error cut off. This error shouldn't be causing a problem when running FastQC since that module is turned off; but I'll update the parameter file in the project so it won't show up.