kingsfordgroup / sailfish

Rapid Mapping-based Isoform Quantification from RNA-Seq Reads
http://www.cs.cmu.edu/~ckingsf/software/sailfish
GNU General Public License v3.0
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index broken #82

Closed butterflyology closed 8 years ago

butterflyology commented 8 years ago

Just installed Sailfish 0.7.5 from the previous version (which worked fine).

Installed the new version, rebuilt the index for my experiment, and now I get:

"[2015-10-05 14:39:23.044] [jointLog] [info] parsing read library format there is 1 lib Exception : [Error: The index version file Sail-0.3-index/versionInfo.json doesn't seem to exist. Please try re-building the salmon index.] /home/chamm/Sailfish/bin/sailfish quant was invoked improperly. For usage information, try /home/chamm/Sailfish/bin/sailfish quant --help Exiting."

I try rebuilding the index, including using --force and just deleting the directory with the index, and I still get this error. When I build the new index, it seems to have far less output then before. I hope this helps.

Chris

rob-p commented 8 years ago

Hi Chris,

When you say "previous" version, do you mean v0.6.3? One thing that has changed (and I will update the documentation to make this more clean) is that the k value you provide to the indexer must be an odd value. That means, you can't say e.g. -k 18 or -k 20. Rather, -k 19 or -k 21 should work. It should complain if you give an even value, so I'll double-check that it does that.

--Rob

P.S. You can actually run the indexer now without a -k argument, and it will assume a default value.

butterflyology commented 8 years ago

Hi Rob,

Thanks for your answer. Changing the K value returned the index functionality. Thanks.

Chris

rob-p commented 8 years ago

No problem :).