kirchhausenlab / incasem

Automated Segmentation of cellular substructures in Electron Microscopy
BSD 3-Clause "New" or "Revised" License
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issue for gc_segment and data transformation(conver tiff to zarr) #23

Open jinxsfe opened 3 months ago

jinxsfe commented 3 months ago

image image image my data volume is 464464140, and the interest Z stack range that I want to input is from stack 24 to stack 110, but stack list is out of range.

But if I use single slice of 3D volume, and I can open GUI smoothly, the two kind of dataset that I put in each folder, the single slice only contain one slice of 3D volume, and volume folder is one 464464140’s single volume. When processing 3D data, do I need seperate 3D volume into individual slices? For example, if z stack is 140, so I will seperate them into 140 single tiff images in volume stack?

Question 2: as you said I should change the configure file in fit small data dimension instead of 204^3, so the configure file is in train.py?

Q3 I try to transfer my dataset image for 40 of each to zarr. and when I processing the code, the result is only one image and I check the structure of these data, seems is image I don't understand why have two "0" folder, and image this is coding running progress

jinxsfe commented 3 months ago

@patrickstock @