Open umasstr opened 1 year ago
it seems the mcmc runs are only 5? if that's the case, you should run more mcmcs, say 700 or above.
On Sat, Apr 29, 2023 at 8:05 PM GitDog @.***> wrote:
To test CDSeq, I used counts from published bulk RNA-seq of human skin (accession numbers below). Gene-level counts were used as input. Surprisingly, estProp from the output always distributes the proportion of single cells evenly across the types. the below command runs to completion. Is there a reason that this might happen? A similar result is also returned with subsets of these samples.
cdsrr<-CDSeq(bulk_data = srr, cell_type_number = 10, mcmc_iterations = 5, # increase the mcmc_iterations to 700 or above gene_length = as.vector(length), cpu_number = 6)
SRR12330925 SRR12330926 SRR12330927 SRR12330928 SRR12330929 SRR12330930 SRR12330931 SRR12330932 SRR12330933 SRR12330947 SRR12330948 SRR12330949 SRR12330950 SRR12330951 SRR12330952 SRR12330953
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Thanks for your reply. I repeated the above command, setting the mcmc_iterations argument = 700 and still get the same results. I also tried setting cell_type_number = 5:10, which produced 5 celltypes, all with the exact same proportions
what is the total counts for each bulk? say what is colSums(srr)?
To test CDSeq, I used counts from published bulk RNA-seq of human skin (accession numbers below). Gene-level counts were used as input. Surprisingly, estProp from the output always distributes the proportion of single cells evenly across the types. the below command runs to completion. Is there a reason that this might happen? A similar result is also returned with subsets of these samples.
SRR12330925 SRR12330926 SRR12330927 SRR12330928 SRR12330929 SRR12330930 SRR12330931 SRR12330932 SRR12330933 SRR12330947 SRR12330948 SRR12330949 SRR12330950 SRR12330951 SRR12330952 SRR12330953