Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found

[HumbiFred]

Hi,

I am struggeling to get Kleborate working. I have installed the required packages and respective versions (biopython, mash, blast+) into a conda environment. Then I have followed the installation manual:

git clone --recursive https://github.com/katholt/Kleborate.git cd Kleborate/kaptive git pull https://github.com/katholt/Kaptive master cd .. ./kleborate-runner.py -h

Everything seemed fine so far. However, when I tried to run kleborate (kleborate -a *fasta -o TEST --all) I always receive the following error:

strain species ST virulence_score resistance_score Yersiniabactin YbST Colibactin CbST Aerobactin AbST Salmochelin SmST RmpADC RmST rmpA2 wzi K_locus K_locus_confidence O_locus O_locus_confidence AGly_acquired Col_acquired Fcyn_acquired Flq_acquired Gly_acquired MLS_acquired Phe_acquired Rif_acquired Sul_acquired Tet_acquired Tgc_acquired Tmt_acquired Bla_acquired Bla_inhR_acquired Bla_ESBL_acquired Bla_ESBL_inhR_acquired Bla_Carb_acquired Bla_chr SHV_mutations Omp_mutationsCol_mutations Flq_mutations truncated_resistance_hits spurious_resistance_hits Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found Error: Kaptive failed to run with the following error: /usr/users/.conda/envs/kleborate/lib/python3.9/site-packages/Bio/GenBank/init.py:1287: BiopythonParserWarning: The NCBI states double-quote characters like " should be escaped as "" (two double - quotes), but here it was not: 'undecaprenyl-phosphate galactose phosphotransferase" glucose-1-phosphate transferase' warnings.warn( Error: blastn encountered an error: Error: mdb_dbi_open: MDB_NOTFOUND: No matching key/data pair found

Seems to be an blastn-error, however I am not able to figure it out. Do you guys maybe have an explanation?

Thanks for your help in advance

Kind regards

Frederik

[HumbiFred]

Update: Problem is solved. In the beginning I used a newer version of blast+. However, once I have downgraded to v2.7.1. everything works well. Thx