Hi,
I am trying to run the MIST algorithm on my affinity proteomics data. I think I have everything in the right format etc. Upon running the first phase - preprocessing with data.txt and keys.txt files only
LOADING DEPENDENCIES...
Warning message:
package ‘ggplot2’ was built under R version 4.1.1
All required R libraries accounted for.
PREPROCESSING FILES
READING FILES
!!! CHARACTERS FOUND IN unique_pep COLUMN. CONVERTING TO NUMERIC.
FILTERING COMMON CONTAMINANTS
FILTERED 529 CONTAMINANTS
MERGING KEYS WITH DATA
98 TOTAL BAITS DETECTED
98/98 BAITS DETECTED IN DATA FILE
98/98 BAITS DETECTED IN KEYS FILE
CONVERTING TO MATRIX
COLLAPSE FILE IS EMPTY
Remove file is empty
EXCLUSIONS FILE IS EMPTY
QUALITY CONTROL
Saving 7 x 7 in image
MIST
Error in if (length(baits_to_exclude) == 0 & sum(rowSums(abundance)) > :
missing value where TRUE/FALSE needed
Calls: main ... mist.main -> mist.getMetrics -> mist.getSpecificity
Execution halted
I can't work out what's wrong. From google searches of similar error messages, I think it may be something to do with some parameters, but I'm unsure which line (in which script) I need to modify.
Any ideas?
Regards,
Vinay
Hi, I am trying to run the MIST algorithm on my affinity proteomics data. I think I have everything in the right format etc. Upon running the first phase - preprocessing with data.txt and keys.txt files only
I can't work out what's wrong. From google searches of similar error messages, I think it may be something to do with some parameters, but I'm unsure which line (in which script) I need to modify. Any ideas? Regards, Vinay