tabix: error while loading shared libraries: libcrypto.so.1.0.0

[lounaDeO]

Hi!

I install pixy using following conda install commands: conda create --name pixy conda activate pixy conda install --yes -c conda-forge pixy conda install --yes -c bioconda htslib

But when I want to use pixy with this command: pixy --stats pi --vcf allPOP.combineGVCF.filter.4fold.10%NA.PASS.vcf.gz --populations pop.txt --window_size 10000 --n_cores 4

I have the following error:

[pixy] pixy 1.2.7.beta1 [pixy] See documentation at https://pixy.readthedocs.io/en/latest/

[pixy] Validating VCF and input parameters... [pixy] Checking write access...OK [pixy] Checking CPU configuration...OK [pixy] Checking for invariant sites...OK tabix: error while loading shared libraries: libcrypto.so.1.0.0: cannot open shared object file: No such file or directory [pixy] Checking chromosome data...subprocess.CalledProcessError: Command 'tabix -l allPOP.combineGVCF.filter.4fold.10%NA.PASS.vcf.gz' returned non-zero exit status 127.

It seems I have an issue with the tabix command but I don't know how to fix it. Any help would be really appreciated.

Thanks in advance! Louna

[ksamuk]

Hi there! Can you check to see if tabix was successfully installed? For example, try typing which tabix in your pixy conda environment.

[lounaDeO]

Yes, it seems to be installed: which tabix /kingdoms/tools/anaconda3/envs/pixy/bin/tabix

[ksamuk]

Ah, I see the error now. You are encountering the issue described here: https://github.com/bioconda/bioconda-recipes/issues/12100#issuecomment-461466897. They have a solution posted there as well, have a look and see if that solves your problem!

Edit: different link, same problem!

[lounaDeO]

Oh yes it works! thank you very much for your help!

[ksamuk]

Excellent, glad to hear it!