Closed Dazcam closed 4 months ago
Hi @Dazcam,
Unfortunately, there is currently no way to do this as you have described it. The best thing you can do is revisit your dimensionality reduction and/or clustering to try to simplify the resulting lineage structure. For example, choosing a smaller resolution parameter (for graph-based clustering) or smaller number of clusters (for k-means and similar methods) could reduce the likelihood of a spurious branching event.
Alternatively, if the spurious lineage is not too intrusive, you can always continue your analysis and simply choose to focus on the results from the other lineages. For example, if you run tradeSeq
on the resulting trajectory, the results will be broken down by lineage, so you can easily produce lists of genes that are significant along any of your non-spurious lineages.
Hope this helps! Best, Kelly
Hi @kstreet13, thank you for your suggestions. I've opted to remove some cells from the trajectory analyses to get around this. The issue was with a small population of myeloid cells were being linked to the neuronal progenitor population that I designated as the root population. These cell types are from different lineages so this made no biological sense. Slingshot appears to draw a lineage from the designated root population to all possible 'maturer' populations.
One other thing that may be useful, depending on your data, is the omega
argument. If the myeloid cells are reasonably well separated from the other cell populations, then you can allow slingshot
to detect breaks in the trajectory by setting omega = TRUE
. Under the default settings, though, you are correct that it will connect all the input clusters.
Hi there,
How would one go about removing a spurious lineage from a
PseudotimeOrdering
object after runninggetLineages
?Running something like
lin1@metadata$lineages$Lineage4 <- NULL
is insufficient as it doesn't remove all lineage information from the object. For example, runninggetCurves(lin1)
after trying to remove lineage 4 using the above code results the following error:Many Thanks