Closed jfertaj closed 7 years ago
Can you post a full error log here?
This error happened in the cross correlation QC step. While we are looking at it, you can disable it with -no_xcor
.
Here is the full log, let me know if you need it by email or another method, thanks a lot
[DATE] : Sun Apr 30 10:06:21 BST 2017
[HOST] : dhcp210.well.ox.ac.uk
[SCR_NAME] : D24.BDS
[BDS_PARAM] : atac.bds -species hg19 -nth 25 -no_par -no_idr -out_dir D24_noENCODE -title D24_noENCODE -fastq1_1 /home/jfertaj/atac-seq/data/WTCHG_367049_13_1.fastq.gz -fastq1_2 /home/jfertaj/atac-seq/data/WTCHG_367049_13_2.fastq.gz -fastq2_1 /home/jfertaj/atac-seq/data/WTCHG_367049_14_1.fastq.gz -fastq2_2 /home/jfertaj/atac-seq/data/WTCHG_367049_14_2.fastq.gz
== git info
Latest git commit : 037cf436c4088d119a580e63c46eacde89d16433 (Wed Apr 26 20:08:12 2017)
Reading parameters from section (default) in file(/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/default.env)...
== configuration file info
Hostname : dhcp210.well.ox.ac.uk
Configuration file :
Environment file : /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/default.env
== parallelization info
No parallel jobs : true
Maximum # threads : 25
== cluster/system info
Walltime (general) : 5h50m
Max. memory (general) : 7G
Force to use a system : local
Process priority (niceness) : 0
Retiral for failed tasks : 0
Submit tasks to a cluster queue :
Unlimited cluster mem./walltime : false
Info: Environments module not found on your system (e.g. /etc/profile.d/modules.sh). Ignoring shell env. parameters like '-mod'.
== shell environment info
Conda env. : bds_atac
Conda env. for python3 : bds_atac_py3
Conda bin. directory :
Shell cmd. for init. : if [[ -f $(which conda) && $(conda env list | grep bds_atac | wc -l) != "0" ]]; then source activate bds_atac; sleep 5; fi; export PATH=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/.:/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/modules:/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s)
Shell cmd. for init.(py3) : if [[ -f $(which conda) && $(conda env list | grep bds_atac_py3 | wc -l) != "0" ]]; then source activate bds_atac_py3; sleep 5; fi; export PATH=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/.:/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/modules:/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s)
Shell cmd. for fin. : TASKTIME=$[$(date +%s)-${STARTTIME}]; echo "Task has finished (${TASKTIME} seconds)."
== output directory/title info
Output dir. : /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE
Title (prefix) : D24_noENCODE
Reading parameters from section (default) in file(/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/default.env)...
Reading parameters from section (hg19) in file(/home/jfertaj/atac-seq/data/bds_pipeline_genome_data/bds_atac_species.conf)...
== species settings
Species : hg19
Species file : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/bds_atac_species.conf
Species name (WashU browser) : hg19
Ref. genome seq. fasta : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/male.hg19.fa
Chr. sizes file : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/hg19.chrom.sizes
Black list bed : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/wgEncodeDacMapabilityConsensusExcludable.bed.gz
== ENCODE accession settings
ENCODE experiment accession :
ENCODE award RFA :
ENCODE assay category :
ENCODE assay title :
ENCODE award :
ENCODE lab :
ENCODE assembly genome :
ENCODE alias prefix : KLAB_PIPELINE
== report settings
URL root for output directory :
Genome coord. for browser tracks :
== align multimapping settings
# alignments reported for multimapping : 0
== align bowtie2 settings
Bowtie2 index : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/bowtie2_index/male.hg19.fa
Walltime (bowtie2) : 47h
Max. memory (bowtie2) : 12G
== adapter trimmer settings
Maximum allowed error rate for cutadapt : 0.10
Minimum trim. length for cutadapt -m : 5
Walltime (adapter trimming) : 23h
Max. memory (adapter trimming) : 12G
== postalign bam settings
MAPQ reads rm thresh. : 30
Rm. tag reads with str. :
No dupe removal in filtering raw bam : false
Walltime (bam filter) : 23h
Max. memory (bam filter) : 12G
Dup marker : picard
Use sambamba markdup (instead of picard) : false
== postalign bed/tagalign settings
Max. memory for UNIX shuf : 12G
== postalign cross-corr. analysis settings
Max. memory for UNIX shuf : 12G
User-defined cross-corr. peak strandshift : -1
Extra parameters for cross-corr. analysis :
== callpeak macs2 settings
Genome size (hs,mm) : hs
Walltime (macs2) : 23h
Max. memory (macs2) : 15G
== callpeak naiver overlap settings
Bedtools intersect -nonamecheck : false
== callpeak etc settings
# of top peaks to pick up in peak files : 500000
== IDR settings
Append IDR threshold to IDR out_dir : false
== ATAQC settings
TSS enrichment bed : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/ataqc/hg19_gencode_tss_unique.bed.gz
DNase bed for ataqc : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/ataqc/reg2map_honeybadger2_dnase_all_p10_ucsc.bed.gz
Promoter bed for ataqc : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/ataqc/reg2map_honeybadger2_dnase_prom_p2.bed.gz
Enhancer bed for ataqc : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/ataqc/reg2map_honeybadger2_dnase_enh_p2.bed.gz
Reg2map for ataqc : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/ataqc/dnase_avgs_reg2map_p10_merged_named.pvals.gz
Reg2map_bed for ataqc : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/ataqc/reg2map_honeybadger2_dnase_all_p10_ucsc.bed.gz
Roadmap metadata for ataqc : /home/jfertaj/atac-seq/data/bds_pipeline_genome_data/hg19/ataqc/eid_to_mnemonic.txt
Max. memory for ATAQC : 15G
Walltime for ATAQC : 47h
== atac pipeline settings
Type of pipeline : atac-seq
Fastqs are trimmed? : false
Align only : false
# reads to subsample replicates (0 if no subsampling) : 0
# reads to subsample for cross-corr. analysis : 25000000
No pseudo replicates : false
No IDR analysis on peaks : true
No ATAQC (advanced QC report) : false
No Cross-corr. analysis : false
Use CSEM for alignment : false
Smoothing window for MACS2 : 150
DNase Seq : false
IDR threshold : 0.1
Use old trim adapters : false
Force to use ENCODE3 parameter set : false
Force to use ENCODE parameter set : false
== checking atac parameters ...
Checking parameters and data files for ATAQC.
== checking adapters to be trimmed ...
Replicate 1 adapters : automatically detected
Replicate 2 adapters : automatically detected
== checking input files ...
Rep1 fastq (PE) :
/home/jfertaj/atac-seq/data/WTCHG_367049_13_1.fastq.gz
/home/jfertaj/atac-seq/data/WTCHG_367049_13_2.fastq.gz
Rep2 fastq (PE) :
/home/jfertaj/atac-seq/data/WTCHG_367049_14_1.fastq.gz
/home/jfertaj/atac-seq/data/WTCHG_367049_14_2.fastq.gz
Distributing 25 to ...
{1=14, 2=11}
Detected adapter for rep1 (PE) : CTGTCTCTTATA, CTGTCTCTTATA
discarding /opt/miniconda3/bin from PATH
prepending /opt/miniconda3/envs/bds_atac/bin to PATH
use 'source("https://bioconductor.org/biocLite.R")' or
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'BiocInstaller' after library("utils")
################
ChIP data: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.gz
Control data: NA
strandshift(min): -500
strandshift(step): 5
strandshift(max) 1500
user-defined peak shift NA
exclusion(min): 10
exclusion(max): NaN
num parallel nodes: 14
FDR threshold: 0.01
NumPeaks Threshold: NA
Output Directory: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1
narrowPeak output file name: NA
regionPeak output file name: NA
Rdata filename: NA
plot pdf filename: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.plot.pdf
result filename: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc
Overwrite files?: TRUE
Decompressing ChIP file
Loading required package: caTools
Reading ChIP tagAlign/BAM file /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.gz
opened /tmp/user/1001/RtmpdankZ2/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign398a36f07e82
00:01:16.852 ExecutionerLocal 'Local[41]': Tasks [Local[41]] Pending: 0 Running: 1 Done: 0 Failed: 0
| PID | Task state | Task name | Dependencies | Task definition |
| ----- | ----------------- | --------- | ------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| 14662 | running (RUNNING) | xcor rep1 | | if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R);; else RUN_SPP=$(which run_spp.R);; fi; Rscript ${RUN_SPP} -rf; -c=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign. |
done. read 19293694 fragments
ChIP data read length 75
[1] TRUE
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
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use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
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use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
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use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
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use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
Calculating peak characteristics
00:02:17.869 ExecutionerLocal 'Local[41]': Tasks [Local[41]] Pending: 0 Running: 1 Done: 0 Failed: 0
| PID | Task state | Task name | Dependencies | Task definition |
| ----- | ----------------- | --------- | ------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| 14662 | running (RUNNING) | xcor rep1 | | if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R);; else RUN_SPP=$(which run_spp.R);; fi; Rscript ${RUN_SPP} -rf; -c=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign. |
00:03:18.885 ExecutionerLocal 'Local[41]': Tasks [Local[41]] Pending: 0 Running: 1 Done: 0 Failed: 0
| PID | Task state | Task name | Dependencies | Task definition |
| ----- | ----------------- | --------- | ------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| 14662 | running (RUNNING) | xcor rep1 | | if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R);; else RUN_SPP=$(which run_spp.R);; fi; Rscript ${RUN_SPP} -rf; -c=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign. |
00:04:19.898 ExecutionerLocal 'Local[41]': Tasks [Local[41]] Pending: 0 Running: 1 Done: 0 Failed: 0
| PID | Task state | Task name | Dependencies | Task definition |
| ----- | ----------------- | --------- | ------------ | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| 14662 | running (RUNNING) | xcor rep1 | | if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R);; else RUN_SPP=$(which run_spp.R);; fi; Rscript ${RUN_SPP} -rf; -c=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign. |
Error in if ((crosscorr$rel.phantom.coeff >= 0) & (crosscorr$rel.phantom.coeff < :
missing value where TRUE/FALSE needed
Execution halted
Minimum cross-correlation value 0.1155747
Minimum cross-correlation shift 1500
Top 3 cross-correlation values NA
Top 3 estimates for fragment length NA
Window half size NA
Phantom peak location 65
Phantom peak Correlation 0.4245396
Normalized Strand cross-correlation coefficient (NSC) NA
Relative Strand cross-correlation Coefficient (RSC) NA
Task failed:
Program & line : 'modules/postalign_xcor.bds', line 121
Task Name : 'xcor rep1'
Task ID : 'atac.bds.20170430_100625_726/task.postalign_xcor.xcor_rep1.line_121.id_10'
Task PID : '14662'
Task hint : 'if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R);; else RUN_SPP=$(which run_spp.R);; fi; Rscript ${RUN_SPP} -rf; -c=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.'
Task resources : 'cpus: 14 mem: -1.0 B wall-timeout: 8640000'
State : 'ERROR'
Dependency state : 'ERROR'
Retries available : '1'
Input files : '[/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.gz]'
Output files : '[/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc, /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.plot.pdf]'
Script file : '/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/atac.bds.20170430_100625_726/task.postalign_xcor.xcor_rep1.line_121.id_10.sh'
Exit status : '1'
Program :
# SYS command. line 123
if [[ -f $(which conda) && $(conda env list | grep bds_atac | wc -l) != "0" ]]; then source activate bds_atac; sleep 5; fi; export PATH=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/.:/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/modules:/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/utils:${PATH}:/bin:/usr/bin:/usr/local/bin:${HOME}/.bds; set -o pipefail; STARTTIME=$(date +%s)
# SYS command. line 126
if [[ $(which run_spp_nodups.R 2> /dev/null | wc -l || echo) == "1" ]]; then RUN_SPP=$(which run_spp_nodups.R); \
else RUN_SPP=$(which run_spp.R); \
fi
# SYS command. line 132
Rscript ${RUN_SPP} -rf \
-c=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.gz -p=14 \
-filtchr=chrM -savp=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.plot.pdf -out=/home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc
# SYS command. line 135
sed -r 's/,[^\t]+//g' /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc > /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc.tmp
# SYS command. line 136
mv /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc.tmp /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc
# SYS command. line 138
TASKTIME=$[$(date +%s)-${STARTTIME}]; echo "Task has finished (${TASKTIME} seconds)."
StdErr (100000000 lines) :
discarding /opt/miniconda3/bin from PATH
prepending /opt/miniconda3/envs/bds_atac/bin to PATH
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'source("http://bioconductor.org/biocLite.R")' to update
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Loading required package: caTools
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
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'source("http://bioconductor.org/biocLite.R")' to update
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'source("http://bioconductor.org/biocLite.R")' to update
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use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
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'source("http://bioconductor.org/biocLite.R")' to update
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'source("http://bioconductor.org/biocLite.R")' to update
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use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
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'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
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'BiocInstaller' after library("utils")
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'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
use 'source("https://bioconductor.org/biocLite.R")' or
'source("http://bioconductor.org/biocLite.R")' to update
'BiocInstaller' after library("utils")
Error in if ((crosscorr$rel.phantom.coeff >= 0) & (crosscorr$rel.phantom.coeff < :
missing value where TRUE/FALSE needed
Execution halted
StdOut (100000000 lines) :
################
ChIP data: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.gz
Control data: NA
strandshift(min): -500
strandshift(step): 5
strandshift(max) 1500
user-defined peak shift NA
exclusion(min): 10
exclusion(max): NaN
num parallel nodes: 14
FDR threshold: 0.01
NumPeaks Threshold: NA
Output Directory: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1
narrowPeak output file name: NA
regionPeak output file name: NA
Rdata filename: NA
plot pdf filename: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.plot.pdf
result filename: /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/qc/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.cc.qc
Overwrite files?: TRUE
Decompressing ChIP file
Reading ChIP tagAlign/BAM file /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.gz
opened /tmp/user/1001/RtmpdankZ2/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign398a36f07e82
done. read 19293694 fragments
ChIP data read length 75
[1] TRUE
Calculating peak characteristics
Minimum cross-correlation value 0.1155747
Minimum cross-correlation shift 1500
Top 3 cross-correlation values NA
Top 3 estimates for fragment length NA
Window half size NA
Phantom peak location 65
Phantom peak Correlation 0.4245396
Normalized Strand cross-correlation coefficient (NSC) NA
Relative Strand cross-correlation Coefficient (RSC) NA
Fatal error: atac.bds, line 941, pos 4. Task/s failed.
Creating checkpoint file: Config or command line option disabled checkpoint file creation, nothing done.
It very hard to figure it out with a log only, can you email (leepc12 at gmail dot com) me your /home/jfertaj/atac-seq/tools/atac_dnase_pipelines/D24_noENCODE/align/rep1/WTCHG_367049_13_1.trim.PE2SE.nodup.25M.tagAlign.gz?
fixed in the commit e929fc8. please get the latest pipeline and try again
Hi,
I have been running a few ATAQ files through your pipeline and all of them have run with no errors but one sample with 2 technical replicates, here is the command i have used
bds_scr D24 atac.bds -species hg19 -nth 25 -no_par -out_dir D24_noENCODE -title D24_noENCODE -fastq1_1 /home/jfertaj/atac-seq/data/WTCHG_367049_13_1.fastq.gz -fastq1_2 /home/jfertaj/atac-seq/data/WTCHG_367049_13_2.fastq.gz -fastq2_1 /home/jfertaj/atac-seq/data/WTCHG_367049_14_1.fastq.gz -fastq2_2 /home/jfertaj/atac-seq/data/WTCHG_367049_14_2.fastq.gz
The program runs until the QC step when I got the following error:
Error in if ((crosscorr$rel.phantom.coeff >= 0) & (crosscorr$rel.phantom.coeff < : missing value where TRUE/FALSE needed
Fatal error: atac.bds, line 941, pos 4. Task/s failed.
Creating checkpoint file: Config or command line option disabled checkpoint file creation, nothing done.
Any ideas?