kundajelab / atac_dnase_pipelines

ATAC-seq and DNase-seq processing pipeline
BSD 3-Clause "New" or "Revised" License
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No adapter trimming? #74

Closed jusu-E404 closed 6 years ago

jusu-E404 commented 6 years ago

Hi guys, I've just updated the pipeline and testing on one of my previously analyzed samples to be sure all works ok - noticed my adapters are not getting trimmed... == checking adapters to be trimmed ... Rep1 R1 adapters (PE) : 00: no adapter specified. Rep1 R2 adapters (PE) : 00: no adapter specified. Rep2 R1 adapters (PE) : 00: no adapter specified. Rep2 R2 adapters (PE) : 00: no adapter specified.

The pipeline just proceeds to straight to bowtie mapping.. Thank you!

leepc12 commented 6 years ago

There was an announcement about this in the google group discussion channel a few weeks ago.

https://groups.google.com/forum/#!topic/klab_genomic_pipelines_discuss/NQ8cZdQZMo8

Please use -auto_detect_adapter.

Thanks,

Jin

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On Tue, Oct 17, 2017 at 10:01 AM, PocketChangeJ notifications@github.com wrote:

Hi guys, I've just updated the pipeline and testing on one of my previously analyzed samples to be sure all works ok - noticed my adapters are not getting trimmed... == checking adapters to be trimmed ... Rep1 R1 adapters (PE) : 00: no adapter specified. Rep1 R2 adapters (PE) : 00: no adapter specified. Rep2 R1 adapters (PE) : 00: no adapter specified. Rep2 R2 adapters (PE) : 00: no adapter specified.

The pipeline just proceeds to straight to bowtie mapping.. Thank you!

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jusu-E404 commented 6 years ago

Oh! Great, thank you! Guess need to catch up on the reading. Thanks again!