Prosit offers high quality MS2 predicted spectra for any organism and protease as well as iRT prediction. When using Prosit is helpful for your research, please cite "Gessulat, Schmidt et al. 2019" DOI 10.1038/s41592-019-0426-7
Hello,
I thank you very much for this super tool but I have (maybe) a stupid question... When I import my predicted library in Skyline which iRT do I must use ? With my experimental library I have a mix between endogeneous iRT peptides and biognosys iRT peptides. Do I have to do a retention time calibration with this predicted library ? If yes with which peptides (biognosys or others) ??
Thank you ! :)
Hello, I thank you very much for this super tool but I have (maybe) a stupid question... When I import my predicted library in Skyline which iRT do I must use ? With my experimental library I have a mix between endogeneous iRT peptides and biognosys iRT peptides. Do I have to do a retention time calibration with this predicted library ? If yes with which peptides (biognosys or others) ?? Thank you ! :)