Hello,
An error occurred when trying to plot the ref ion image after normalization using the "reference" method.
Error in image.default(1, vals, t(as.matrix(vals)), col = col, xaxt = "n", :
increasing 'x' and 'y' values expected
A possible reason is that the normalization creates a reference ion vector with the same value. And the color scale failed to create a color range pallet for this.
MASHUOA
commented
3 years ago
Hello, An error occurred when trying to plot the ref ion image after normalization using the "reference" method.
Error in image.default(1, vals, t(as.matrix(vals)), col = col, xaxt = "n", : increasing 'x' and 'y' values expected
A possible reason is that the normalization creates a reference ion vector with the same value. And the color scale failed to create a color range pallet for this.
range(imdata_ed@imageData$data$intensity[ref_ion,])
c(1,1)
For a null feature, the vector may only contain the zero value which can be treated as a blank ion image.