Open CristiSoitu opened 1 year ago
We would have to see the image data to diagnose this sort of problem. Would you be willing to share it privately? You can reach out to the email address in my profile.
Be aware that stitching several Z slices of the same sample independently is going to introduce the same kind of error as stitching cycles independently. In our own lab we stitch datasets with Z stacks by stitching one slice and then applying the corrected tile positions to the other stacks. The Ashlar command line tool doesn't yet support saving the corrected positions after stitching or reusing them for later registration, but I could help write a small python script to achieve this if you are interested.
Hello,
I'll start by saying that this is a great, well-needed resource for the community. I'm posting these issues here, since it may be relevant for other people too. I have encountered 2 sorts of issues while using ashlar:
Cristian