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laurahspencer / DuMOAR

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Get RNA quality information from sequencing facility from library prep #34

Closed laurahspencer closed 10 months ago

laurahspencer commented 1 year ago

@mgavery @kristamnichols can either of you track down the RNA quality determined by the sequencing facility during extraction / library prep?

mgavery commented 1 year ago

@kristamnichols, we think this was UO who did this sequencing. Could you log in and see the QC report?

kristamnichols commented 1 year ago

QC reports for RNA sample qc -30-544518807-1 to 24_3f9d4531-fc48-414b-b001-27939ce803c2.pdf Sample QC report of_30-544518807_210715022328.pdf

mgavery commented 1 year ago

Thanks! Those RIN values are pretty low, but I don't have a lot of experience with low RINs to know impact on library quality though. @kristamnichols , do you know how they link the sample description ID in the report with our sample IDs? I'm wondering if any correlation between mapping rate and RIN

kristamnichols commented 1 year ago

@mgavery sample description is the 'genewiz id' in the other PDF, which links it to our sample IDs

mgavery commented 1 year ago

Thanks, I must have been clicking the same link over an over. Ha! I didn't see the second report