Error in clustree.matrix(clusterings, prefix, metadata = metadata, ...) : Assertion on 'x' failed: Contains missing values (row 23366, col 1).

[LiHangzls]

this is code: pbmc_list %>% RunUMAP(dim=1:20) %>% FindNeighbors(dim=1:20) %>% FindClusters(resolution =c(0.1,0.2,0.4,0.6,0.8))-> pbmc_list library(clustree) pbmc_list@meta.data %>% dplyr::select(starts_with("integrated_snn_res")) %>% dplyr::mutate(integrated_snn_res.0.0=0)%>% clustree( prefix = "integrated_snn_res") this is error: Error in clustree.matrix(clusterings, prefix, metadata = metadata, ...) : Assertion on 'x' failed: Contains missing values (row 23366, col 1).

[lazappi]

It's a bit hard to say why from the provided code but the error suggests there is a missing value in the provided data. Please check you input data to see why there are missing values.

[lazappi]

Closing this issue but please reopen if you have further questions