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lehner-lab / DiMSum

An error model and pipeline for analyzing deep mutational scanning (DMS) data and diagnosing common experimental pathologies
MIT License
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invalid 'nt_seq' values #19

Closed mingbao96 closed 6 months ago

mingbao96 commented 6 months ago

Hi,

I'm running the DiMSum to process the count files generated in other ways. When a variant count file was fed, an ERROR happened:

Error: One or more invalid 'nt_seq' values in variant count file specified by 'countPath'. \
Only valid nucleotide sequences allowed (A/C/T/G).

Execution halted

But the sequences are all composed of a/c/t/g, so it is confusing. How was it going, do you have any ideas?

Best wishes

mingbao96 commented 6 months ago

I got it, there are some N bases