We performed a deep sequencing experiment of a mutational library created with error prone PCR. We are focused on a single gene and have 50 million reads in the INPUT and selection sample. I'm attaching the output from the run. I am using version 1.3.1 and R version 4.0.5 and installing via Conda and running on a ScentOS 7 system.
Everything runs fine until Stage 4 when I get the following error:
******* DiMSum STAGE 4 (STEAM): PROCESS VARIANT SEQUENCES *******
Loading variant count files:
./TH02_DiMSum_output/tmp/3_tally/TH01_e1_s0_bNA_tNA.vsearch.unique
./TH02_DiMSum_output/tmp/3_tally/TH02_e1_s1_b1_tNA.vsearch.unique
Processing...
TH01_e1_s0_bNA_tNA.vsearch.unique
TH02_e1_s1_b1_tNA.vsearch.unique
Processing merged variants...
Error: Cannot proceed with variant processing: No substitution variants found
Execution halted
The sequencing files are Illumina short read sequences for the entire plasmid construct, but I am only providing the WT sequence for the gene of interest (minus the STOP codon). Any help getting this to run would be greatly appreciated!
We performed a deep sequencing experiment of a mutational library created with error prone PCR. We are focused on a single gene and have 50 million reads in the INPUT and selection sample. I'm attaching the output from the run. I am using version 1.3.1 and R version 4.0.5 and installing via Conda and running on a ScentOS 7 system.
Everything runs fine until Stage 4 when I get the following error:
The sequencing files are Illumina short read sequences for the entire plasmid construct, but I am only providing the WT sequence for the gene of interest (minus the STOP codon). Any help getting this to run would be greatly appreciated!
DiMSum_log.txt