lehtiolab / ddamsproteomics

A Nextflow MS DDA proteomics pipeline
MIT License
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Enforce concatenated search for percolator #26

Closed glormph closed 10 months ago

glormph commented 1 year ago

Percolator auto-detects if search is separate t/d or concatenated. Since it does so by finding duplicate scan numbers, we get problems when using multiple fractions, leading to percolator using the mix-max algo.

We don't want that, and to enforce it we should use -Y. Likely get better scores as well.