Open gsc74 opened 2 years ago
A GFA to rGFA tool would be extremely helpful for my use case at the moment. At a minimum, I could use more information about how the SO value is calculated; it's not 100% clear to me, and trying to determine what it should be while parsing the reference human pangenome freeze 1 (which only has intermittent SO tags) has proven to be unexpectedly complex!
I think the reverse is true, a rGFA to GFA
conversion tool is (also) severely lacking.
While minigraph is apparently the most efficient and promising pangenome tool at present, the use of rGFA prohibits downstream analysis using the odgi and vg toolkits (at least to my knowledge). Therefore, no SNP calling, odgi pavs calling etc is possible.
Has there been any progress made on this type of tool, either in gfatools
or other toolchain?
I think at present in 2024, I'd recommend creating new pangenomic graphs using Minigraph-cactus
to get GFA
format output (if possible).
There is discussion on this topic here too - https://www.biostars.org/p/9601440/#9601480
There is still a lot of room for improvement in the pangenomic tool GFA
and rGFA
landscape(s).
@lh3, I'm curious to know , whether we can convert
GFA
torGFA
? . As per rGFA format, we need to maintain 3 additional information along with each segment lines (S
). So, Assuming we haveGFA
with segments links;can't we just add ;
Will it be valid
rGFA
?