Closed chklopp closed 1 year ago
Could you explain how the rank found in the gff format is calculated? What is the frameshift weight? In my test file the rank 1 alignment has not the best Identity or Positive value, but it still corresponds to the reference gene location.
It is ranked by the Smith-Waterman alignment score without considering introns.
Could you explain how the rank found in the gff format is calculated? What is the frameshift weight? In my test file the rank 1 alignment has not the best Identity or Positive value, but it still corresponds to the reference gene location.