Open zhaijj opened 1 year ago
What have you tried? How many exons does the gene have? Tblastn doesn't do splice alignment.
I tried to lower all gap penalty with the following command:
miniprot -O 1 -J 1 -F 1 -N 1000 --outn=1000
I also tried -S
to do alignment without splicing, but it returns the same alignment.
Only one CDS (two exons, the first one was UTR) in my query gene.
Hi,
I managed to get very high sensitivity with the following parameters: -k 3 -L 5 -O 5 -n 1 -N 1000 -l 3 -E 0 -J 5 -F 8 -B 5 --outs 0.5 --outn 1
I think key parameters that you did not use are -n
-k
-l
and mostly --outs
, I suggest that you use --outc also in your case.
However in my case having one and only one mapping per query was a good assumption (I was mapping metapneumovirus proteins on Flu 1 genome). I was just doing that to evaluate the tool capacity to annotate unknown viruses (which is good).
Hi,
I was using miniprot to align one protein in Sorghum to maize genome, but no matter how I changed the parameters, only one alignment was returned. But if I used tblastn with default parameter, it can return 12 alignments.
So could you point out to me which parameters are responsible for the sensitivity of miniprot?