I tried to run this command: samtools mpileup -C50 -uf ref.pri_genomic.fna A_41_sorted.bam | bcftools view -c - | vcfutils.pl vcf2fq -d10 -D100 | gzip > A_41_psmc.fq.gz
I tried different commands, but always the following error message appears:
Could not parse --min-ac -
Use of uninitialized value $l in numeric lt (<) at /opt/apps/software/BCFtools/1.10.2-GCC-8.3.0/bin/vcfutils.pl line 566.
Use of uninitialized value $l in numeric lt (<) at /opt/apps/software/BCFtools/1.10.2-GCC-8.3.0/bin/vcfutils.pl line 566.
I used CentOS 7.8.2003
samtools/0.1.19
bcftools/1.10.2
samclip/0.2
Dear lh3,
I tried to run this command: samtools mpileup -C50 -uf ref.pri_genomic.fna A_41_sorted.bam | bcftools view -c - | vcfutils.pl vcf2fq -d10 -D100 | gzip > A_41_psmc.fq.gz
I tried different commands, but always the following error message appears: Could not parse --min-ac - Use of uninitialized value $l in numeric lt (<) at /opt/apps/software/BCFtools/1.10.2-GCC-8.3.0/bin/vcfutils.pl line 566. Use of uninitialized value $l in numeric lt (<) at /opt/apps/software/BCFtools/1.10.2-GCC-8.3.0/bin/vcfutils.pl line 566.
I used CentOS 7.8.2003 samtools/0.1.19 bcftools/1.10.2 samclip/0.2
I am not sure what else I can do.
Thanks in advance.