Lost 2 lines of my fastq file when reverse complementing the file

lh3 / seqtk

Toolkit for processing sequences in FASTA/Q formats

MIT License

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Closed senaj closed 6 years ago

senaj commented 6 years ago

command: seqtk seq -r forward.fq > rc_forward.fq

Line count of the input and output files

wc -l *fq

63008 forward.fq 63006 rc_forward.fq

shenwei356 commented 6 years ago

You may upload some reads to reproduce the result.

lh3 commented 6 years ago

Yes, it would be good to upload an example. You may first try unix command "tail" on both files to check if the last record is correct.

lh3 commented 6 years ago

Close due to lack of information. Please reopen when have more information.