Closed aungthurhahein closed 6 years ago
I was trying to get subsequences but the result doesn't show up anything.
ID File:
@NB501274:11:HG3WCAFXX:1:11101:10000:11203 @NB501274:11:HG3WCAFXX:1:11101:10000:8239 @NB501274:11:HG3WCAFXX:1:11101:10001:15495
Orginial Fastq file:
@NB501274:11:HG3WCAFXX:1:11101:16414:1046 1:N:0:ATCACG TAAGCTAATGCCGGTGTAAAGTGTTGAGTCCCTTAGTCAATCTCCAAGAGCCGTGTAGCCCTGAACTGGGGTCCC + AAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEAE @NB501274:11:HG3WCAFXX:1:11101:13913:1047 1:N:0:ATCACG GGCAAACGTCTTCGCCGAGGTGACACACCGAAGAAGCCCAATCGCAGTAGTAGCTCTGGGGGTTGTACAGCGTTC + AAAAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEE @NB501274:11:HG3WCAFXX:1:11101:13331:1048 1:N:0:ATCACG TGGAGAACGATGCGGCCACCTCGCTTGTTCTGGTACTTCATGAAAATCTGGGCATGTTCCCTTTCCTCATCGCTC + AAAAEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEE @NB501274:11:HG3WCAFXX:1:11101:11057:1049 1:N:0:ATCACG GCCTGTGGTATCCAGGTAGGTGTAGTGCAGACTGTCAGCTTTGCGCTCTACTGGGTATGGTGTCTCAAGATCAAT +
CMD: seqtk subseq R1.fq test.lst
seqtk subseq R1.fq test.lst
Remove @ in ID file.
@
sed 's/^@//' id.txt > new.txt
Thanks, @shenwei356!
To @aungthurhahein: as @shenwei356 said, you need to remove the leading @ character.
I was trying to get subsequences but the result doesn't show up anything.
ID File:
Orginial Fastq file:
CMD:
seqtk subseq R1.fq test.lst