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lh3 / seqtk

Toolkit for processing sequences in FASTA/Q formats
MIT License
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set seed with different samples #154

Closed kaiwang12 closed 4 years ago

kaiwang12 commented 4 years ago

Hi, I'm not sure how seqtk set up the seed. I have 20 samples, and each sample has 2 paired read files (forward and reverse). The smallest file only has 10,011 reads, the largest file has 50,032 reads. So I wanted to subsample all of my samples to this level (10,000).

Do I have to change -s parameter for different samples, or should I use the same seed?

Thanks and any wisdom would be appreciated.

lh3 commented 4 years ago

It doesn't matter. Either way works.