Closed demis001 closed 8 years ago
It output the quality on the seq line and give the quality on the separate again. The quality next to the sequence should be removed to be a correct format. Any fix? version 1.1
seqtk trimfq -b 17 -e 17 outdir/input/seqWithWallBarcodeCombined.fastq >out_trim.fastq
@HISEQ:111:IHHVDQKLL:1:1000:900:20000 1:N:0:CATATTG GGCTTTTGAGGATACCCCGGTCTCCCAAATAAATACATTCATCTGTAAAGATCGGAAGAGCACACCATATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO + AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO @HISEQ:111:OJWOAZKVB:1:1000:900:20000 1:N:0:CATATTG GGCTTTTGAAGGCCAGGACTCGTTTGTACCCGTTGATGATAGAATGGGCGATCGGAAGAGCACACTATATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO + AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO
It is a bug in 1.1. Please use the latest master branch. Thanks.
It output the quality on the seq line and give the quality on the separate again. The quality next to the sequence should be removed to be a correct format. Any fix? version 1.1
seqtk trimfq -b 17 -e 17 outdir/input/seqWithWallBarcodeCombined.fastq >out_trim.fastq
@HISEQ:111:IHHVDQKLL:1:1000:900:20000 1:N:0:CATATTG GGCTTTTGAGGATACCCCGGTCTCCCAAATAAATACATTCATCTGTAAAGATCGGAAGAGCACACCATATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO + AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO @HISEQ:111:OJWOAZKVB:1:1000:900:20000 1:N:0:CATATTG GGCTTTTGAAGGCCAGGACTCGTTTGTACCCGTTGATGATAGAATGGGCGATCGGAAGAGCACACTATATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO + AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAaO