lh3 / seqtk

Toolkit for processing sequences in FASTA/Q formats
MIT License
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SEQTK: Convert illumina1.5 to illumina 1.9 #77

Open muhammadsohailraza opened 8 years ago

muhammadsohailraza commented 8 years ago

Hi, I am trying to utilize seqtk in order to convert quality score encoding of raw FASTQ files from illumina 1.5 to Sanger / illumina 1.9 my command line was:

seqtk seq -VQ64 inputfile-illumina1.5.fq.gz > outputfile-illumina1.9.fq.gz

I run this command for more than 24 hours , but no output.. is there any mistake in command line??

kind regards sohail

BIG, CAS, Beijing , China

tseemann commented 8 years ago

@muhammadsohailraza

You seem to be mixing two separate options -V and -Q xx ?