Since yak count requires two identical streams for paired end reads, is it necessary to have forward/reverse reads separated by file or labeled as /R1 /R2? I'm regularly converting paired end short read bams -> fasta for yak count and wondered if I need to bother sorting and separating reads by R1/R2.
Since yak count requires two identical streams for paired end reads, is it necessary to have forward/reverse reads separated by file or labeled as /R1 /R2? I'm regularly converting paired end short read bams -> fasta for yak count and wondered if I need to bother sorting and separating reads by R1/R2.