Thanks a lot for providing a powerful tool to analyze spike-in chip-seq data. In your paper, I noticed that you always use only one antibody (such as histone modification), however, recently we performed spike-in chip-seq experiment using two antibodies, one (transcription factor) for human, and the other (H2Av, drosophila-specific antibody) for drosophila. Hence, I wondered whether Spiker could be utilized to analyze our data.
You can still use this pipeline to analyze your data, although I don't know the advantage of using two antibodies (the human TF not expressed in fly?).
Hi developers,
Thanks a lot for providing a powerful tool to analyze spike-in chip-seq data. In your paper, I noticed that you always use only one antibody (such as histone modification), however, recently we performed spike-in chip-seq experiment using two antibodies, one (transcription factor) for human, and the other (H2Av, drosophila-specific antibody) for drosophila. Hence, I wondered whether Spiker could be utilized to analyze our data.