Open seunghun23 opened 11 months ago
Could you please use zcat to check whether the fastq files are in the right path and the format is correct?
Hi,
I tried zcat using the same path I used for the fastq files and they are in the right path with right format. I used the same fastq files to run MixCR without any problem
Do you have the write permission to the folder /mnt/TCR/TRUST4?
I do have the write permission.
Could you please run the example files provided in TRUST4 package, just to make sure the installation is fine? Thank you.
Could you please run the example files provided in TRUST4 package, just to make sure the installation is fine? Thank you.
Hi, I have the same error info when I run the example files.
@jiqingxiaoxi @seunghun23 Thank you for letting me know about this issue. I think this is a bug from the latest git push. I'll fix it in the next a few hours or tomorrow. In the meantime, you can use the v1.0.12 package on the github release page. Thank you!
Sorry for writing too many messages. I think I've fixed the issue, and pushed the new code to the github repo. Could you please pull the new repo, recompile TRUST4, and give it a try? Thank you.
@mourisl Thank you for the quick fix. Looks like fastq-extractor is now working correctly without the above mentioned error. I will let you know if I encounter any other problem with this version.
Hi @mourisl I downloaded TRUST4 today and tried to run the example fastq file. It did not work and I got similar error message. The error messagge is as follows: failed: -1 at ./run-trust4 line 54. I really appreciate it, if you can help me fixing this bug. Thank you so much.
Could you please show me the full output on the screen by TRUST4?
The following is the command that I typed and the error message: $ ./run-trust4 -f human_IMGT+C.fa --ref human_IMGT+C.fa -1 example/example_1.fq -2 eample/example_2.fq -o TRUST_example [Tue Jan 9 15:15:51 2024] TRUST4 begins. [Tue Jan 9 15:15:51 2024] SYSTEM CALL: /c/Users/YB/Desktop/TRUST4/astq-extractor -t 1 -f human_IMGT+C.fa -o TRUST_example_toassemble -1 example/exampe_1.fq -2 example/example_2.fq Can't exec "/c/Users/YB": No such file or directory at ./run-trust4 line 54. system /c/Users/YB/Desktop/TRUST4/fastq-extractor -t 1 -f human_IMG+C.fa -o TRUST_example_toassemble -1 example/example_1.fq -2 example/example_2.fq filed: -1 at ./run-trust4 line 54.
Some how your message is missing some character, for example, "fastq-extractor" is "astq-extractor" ,and "/c/Users/YB" seems incomplete too.
Have you run "make" to compile TRUST4 first, i.e. do you see executable files like fastq-extractor in the folder? I never tested TRUST4 on Windows, so I'm not sure how it will work out.
I thought that i compiled TRUST4 just now, but apparently it was not complete. The command "make" could not compile TRUST4 completely. I am trying to figure out how to do this first.
I am almost done with compiling TRUST4. I had to excute the commands one by one since "make" did not work for some reasons. I am almost done with the compilation, I believe. I just got stuck at 2 steps which involve: bgzf.c and knetfile.c.
the problem related to bgzf.c is the following:
when I typed: g++ -o bam-extractor -I./samtools-0.1.19 -L./samtools-0.1.19 -O3 -Wall -g BamExtractor.o -lpthread -lz -lbam -lws2_32,
I got the following error messages:
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ./samtools-0.1.19/libbam.a(bgzf.o): in function bgzf_open': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19/bgzf.c:142: undefined reference to
compressBound'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ./samtools-0.1.19/libbam.a(bgzf.o): in function bgzf_dopen': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19/bgzf.c:160: undefined reference to
compressBound'
collect2.exe: error: ld returned 1 exit status
the problem related to knetfile.c is the following:
when i inputted: gcc -g -Wall -O2 -o bcftools call1.o main.o ../kstring.o ../bgzf.o ../knetfile.o ../bedidx.o -L. -lbcf -lm -lz -lpthread
I got the following error messages:
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function socket_connect': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:163: undefined reference to
imp_socket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:164: undefined reference to `imp_setsockopt'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:170: undefined reference to __imp_inet_addr' C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:171: undefined reference to
imp_gethostbyaddr'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:177: undefined reference to `imp_htons'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:178: undefined reference to __imp_connect' C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:167: undefined reference to
imp_gethostbyname'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:164: undefined reference to `imp_WSAGetLastError'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:163: undefined reference to __imp_WSAGetLastError' C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:178: undefined reference to
imp_WSAGetLastError'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:173: undefined reference to `imp_WSAGetLastError'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function socket_wait': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:72: undefined reference to
imp_select'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:79: undefined reference to `imp_WSAGetLastError'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_get_response': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:215: undefined reference to
imp_recv'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function knet_win32_init': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:139: undefined reference to
imp_WSAStartup'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function knet_win32_destroy': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:143: undefined reference to
imp_WSACleanup'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_send_cmd': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:238: undefined reference to
imp_send'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:238: undefined reference to __imp_send' C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:238: undefined reference to
imp_send'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_reconnect': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:284: undefined reference to
imp_closesocket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_send_cmd': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:238: undefined reference to
imp_send'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:238: undefined reference to __imp_send' C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:238: undefined reference to
imp_send'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_connect_file': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:325: undefined reference to
imp_closesocket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_send_cmd': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:238: undefined reference to
imp_send'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_connect_file': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:359: undefined reference to
imp_closesocket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function khttp_connect_file': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:411: undefined reference to
imp_closesocket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:417: undefined reference to `imp_send'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:419: undefined reference to __imp_recv' C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:440: undefined reference to
__imp_closesocket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function my_netread': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:191: undefined reference to
imp_recv'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function kftp_reconnect': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:284: undefined reference to
__imp_closesocket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: ../knetfile.o: in function knet_close': C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:586: undefined reference to
imp_closesocket'
C:/Users/YB/Desktop/mingw64/x86_64-13.2.0/mingw64/bin/../lib/gcc/x86_64-w64-mingw32/13.2.0/../../../../x86_64-w64-mingw32/bin/ld.exe: C:\Users\YB\Desktop\mingw64\TRUST4\samtools-0.1.19\bcftools/../knetfile.c:581: undefined reference to `imp_closesocket'
collect2.exe: error: ld returned 1 exit status
I added:
at the beginning of knetfile.c and also added -lws2_32 at the end of the g++ command, but the command still did not work. I am very grateful if you have any suggestions on how to excute these two commands properly so that I can finish the compilation.
Thank you.
If your input data is in fastq format, you can skip the compiling for the "bam-extractor", which is for bam input file. This may save you some efforts?
Hi, I tried to run TRUST4 on paired end Bulk RNA-Seq Fastq files, but it failed with "139 at ./run-trust4 line 54" as shown in the screenshot below. Could you tell me what this error message means and a way to fix this?
Thanks!![image](https://github.com/liulab-dfci/TRUST4/assets/38226390/b2bf6ec2-9d9a-40cf-97d4-41685db59423)