Closed 322029 closed 5 months ago
Thank you for your prompt response! As for cid, I found some of the same cids separating into a few lines, and their CDR3nt are slightly different. So, I'm interested in the difference between cid and cluster. Would you tell me about this?
cid is the consensus ID, where each consensus sequence may encode multiple CDR3 sequences. They will be automatically in the same cluster.
I understand. Thank you so much!
Thank you for your great tool. I have three questions as for clustering.
As indicated in the title, is it possible to cluster CDR3nt among multiple samples? Although CDR3nt in a sample can be clustered by "trust-cluster.py", inputting multiple trust_report.tsv files is not allowed. I suppose putting together the trust_report.tsv files into a whole and executing trust-cluster.py will work. Is it correct?
Alternatively, trust_report.tsv files provide "cid" (assemble~), and the cids seem to be consistent with other samples'. So, can I substitute cid for cluster?
I'm wondering whether CDR3nt in the same cluster show similar antigenic specificities or their sequences are just similar.
I'd appreciate if you could reply.