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liuzj039 / jpy_tools

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Relationship between expression ratio in/out and expression quantity #3

Open Xoey0314 opened 1 month ago

Xoey0314 commented 1 month ago

Hi Liu,

I attempted to analyze the single-cell sequencing data using joy_tools, and initially, it seemed to proceed smoothly. However, I found myself puzzled by the concept of the expression ratio in/out and how to derive the gene expression quantity based on it.

Best regards, Xoey

liuzj039 commented 1 month ago

Hello, Xoey,

I apologize for any confusion caused by some unclear terms. This repository contains snippets I have used at different times, so the styles are not consistent with each other, which might be confusing for everyone (including myself). Regarding the issue you mentioned, could you provide the code you are using? This would help me understand your concerns more quickly. Based on the literal meaning, I suspect that "expression ratio in" refers to how many cells in a specific cluster show detectable expression of a certain gene, while "out" refers to outside of that cluster. Here is a very simple tutorial that I hope will be helpful to you.

Best regards, zhijian