Dears SqueezeMeta developpers,
I've been trying to analyse my data for several days now but I always get an error on step 10. The first sample passes but when it wants to start the 2nd sample, I always get an error like: the program has finished abnormally.
[34m[6 days, 16 hours, 3 minutes, 6 seconds]: STEP10 -> MAPPING READS: 10.mapsamples.pl
[0m Reading samples from /pasteur/zeus/projets/p02/Biomics/Bioinfo/projet_Ariane/Result/data/00.Result.samples
Metagenomes found: 32
Mapping with Bowtie2 (Langmead and Salzberg 2012, Nat Methods 9(4), 357-9)
Creating reference from contigs
Working with sample 1: B1
Getting raw reads
Aligning to reference with bowtie
Calculating contig coverage
Reading contig length from /pasteur/zeus/projets/p02/Biomics/Bioinfo/projet_Ariane/Result/intermediate/01.Result.lon
Counting with sqm_counter: Opening 36 threads
1626281 reads counted
3252561 reads counted
4878841 reads counted
6505121 reads counted
8131401 reads counted
9757681 reads counted
11383961 reads counted
13010241 reads counted
14636521 reads counted
16262801 reads counted
17889081 reads counted
19515361 reads counted
21141641 reads counted
22767921 reads counted
24394201 reads counted
26020481 reads counted
27646761 reads counted
29273041 reads counted
30899321 reads counted
32525601 reads counted
34151881 reads counted
35778161 reads counted
37404441 reads counted
39030721 reads counted
40657001 reads counted
reads counted
42283281 reads counted
reads counted
reads counted
reads counted
reads counted
reads counted
reads counted
reads counted
reads counted
reads counted
Working with sample 2: B10
Getting raw reads
Aligning to reference with bowtie
[31mStopping in STEP10 -> 10.mapsamples.pl. Program finished abnormally
[0m[31m
Before I was using the previous version, it didn't work and a week ago, I updated the pipline and I'm on the latest version (1.6.2) but I still have the same problem. could you help me solve the problem? thank you for your help.
please find attached the syslog.
We haven't experienced this kind of error in a while now. Please try again with the latest version of Fooocusand feel free to reopen if still relevant.
Dears SqueezeMeta developpers, I've been trying to analyse my data for several days now but I always get an error on step 10. The first sample passes but when it wants to start the 2nd sample, I always get an error like: the program has finished abnormally. [34m[6 days, 16 hours, 3 minutes, 6 seconds]: STEP10 -> MAPPING READS: 10.mapsamples.pl [0m Reading samples from /pasteur/zeus/projets/p02/Biomics/Bioinfo/projet_Ariane/Result/data/00.Result.samples Metagenomes found: 32 Mapping with Bowtie2 (Langmead and Salzberg 2012, Nat Methods 9(4), 357-9) Creating reference from contigs Working with sample 1: B1 Getting raw reads Aligning to reference with bowtie Calculating contig coverage Reading contig length from /pasteur/zeus/projets/p02/Biomics/Bioinfo/projet_Ariane/Result/intermediate/01.Result.lon Counting with sqm_counter: Opening 36 threads 1626281 reads counted 3252561 reads counted 4878841 reads counted 6505121 reads counted 8131401 reads counted 9757681 reads counted 11383961 reads counted 13010241 reads counted 14636521 reads counted 16262801 reads counted 17889081 reads counted 19515361 reads counted 21141641 reads counted 22767921 reads counted 24394201 reads counted 26020481 reads counted 27646761 reads counted 29273041 reads counted 30899321 reads counted 32525601 reads counted 34151881 reads counted 35778161 reads counted 37404441 reads counted 39030721 reads counted 40657001 reads counted reads counted 42283281 reads counted reads counted reads counted reads counted reads counted reads counted reads counted reads counted reads counted reads counted Working with sample 2: B10 Getting raw reads Aligning to reference with bowtie [31mStopping in STEP10 -> 10.mapsamples.pl. Program finished abnormally [0m[31m
Before I was using the previous version, it didn't work and a week ago, I updated the pipline and I'm on the latest version (1.6.2) but I still have the same problem. could you help me solve the problem? thank you for your help. please find attached the syslog.
syslog.zip